Transposition of IS50L activates downstream genes.
نویسندگان
چکیده
A transposition system constructed to detect the transposition of Tn5 to a site upstream of the lacZ gene has revealed that transposition of IS50L can activate downstream genes. Expression is apparently mediated by the NPTII promoter. Transposase produced either by IS50R or by the suppressed IS50L catalyzed transposition of IS50L.
منابع مشابه
A product of the TN5 transposase gene inhibits transposition.
The bacterial transposon Tn5 possesses a regulatory mechanism that allows it to move with higher efficiency when it is first introduced into a cell than after it is established. Tn5 is a composite transposable element containing inverted repeats of two nearly identical elements, IS50R, which encodes the transposase protein necessary for Tn5 movement, and IS50L which contains an ochre mutant all...
متن کاملGain-of-function mutations in TnsC, an ATP-dependent transposition protein that activates the bacterial transposon Tn7.
The bacterial transposon Tn7 encodes five genes whose protein products are used in different combinations to direct transposition to different types of target sites. TnsABC + D directs transposition to a specific site in the Escherichia coli chromosome called attTn7, whereas TnsABC + E directs transposition to non-attTn7 sites. These transposition reactions can also recognize and avoid "immune"...
متن کاملNucleic Acids Research Completion of the nucleotide sequence of the central region of Ta5 confirms the presence of three resistance genes
The DNA sequence of the region located downstream from the kanamycin resistance gene of Tn5_ up to the right inverted repeat TS50R has been determined. This completes the determination of the sequence of Tn5 which is 5818 bp long. The 2.7 Kb central region contains three resistance genes : the kanamycin-neomycin resistance gene, a gene coding for resistance to CT.990 an antimitotic-antibiotic c...
متن کاملPreferential transposition of an IS630-associated composite transposon to TA in the 5'-CTAG-3' sequence.
A composite transposon, Tn4731, associated with IS630 has been shown to transpose preferentially to 5'-TA-3' sequences that are located at two sites in a rho-dependent transcription terminator in plasmid ColE1 in Escherichia coli (T. Tenzen, S. Matsutani, and E. Ohtsubo, J. Bacteriol. 172:3830-3836, 1990). Here we demonstrated that Tn4731 preferentially transposes to TA sequences at four sites ...
متن کاملSTUDY OF HMGA2 GENE INHIBITION WITH SPECIFIC SHRNA AND SIRNA AND INVESTIGATION OF CORRESPONDING EFFECTS ON DOWNSTREAM GENE EXPRESSION IN MDA-MB-231 CANCER CELLS: A BIOINFORMATIC AND EXPERIMENTAL STUDY
Background & Aims: The use of siRNA to silence gene expression is increasingly expanding today. The aim of this study is to bioinformatically and experimentally investigate the inhibition of the HMGA2 gene and its corresponding effects on downstream genes expression rate in MDA-MB-231 cancer cell treated by shRNA and siRNA specific to HMGA2. Materials & Methods: To perform this bioinformatic a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of bacteriology
دوره 170 4 شماره
صفحات -
تاریخ انتشار 1988